Advantages and disadvantages of manual homogenizer and common troubleshooting methods
 May 26, 2021|View:379

The principle of manual homogenizer is to achieve separation, mixing, crushing and homogenization by rotating the pestle and mortar in the homogenizer, which is suitable for handling tough samples.


manual homogenizer


Scope of application of manual homogenizer: It is used to disperse animal/plant tissues, to do extraction of nucleic acid, protein, etc. with lysis solution, and also can be used in industry to make suspension/emulsion of resin and pigment, etc.

Advantages: with low speed, high torque, no noise, etc. Easy to use, by changing different probes, different amounts of samples can be processed, easy to operate and more suitable for single sample operation.

Disadvantages: can not handle multiple samples at the same time, different samples need to change or clean the probe, increasing the chance of cross-contamination between samples; for the processing of thick-walled samples such as bacteria, yeast and other fungi do not consider this type of homogenizer.

Common failure problems and solutions of manual homogenizer.

1. The machine can not start

(1) Electricity or line is not working. Need to check the main motor power installation line is not faulty.

(2) If the pipeline is equipped with automatic safety control system, it is necessary to check whether the system is feeding or discharging failure.

2. The main motor reverses, that is, the main motor does not match with the specified steering, just change the ground wire of the three-phase motor to one phase.

3. Transmission box sound abnormal, the causes and methods of elimination

(1) Check whether the oil level in the transmission is normal, if the oil is not enough to refuel to the oil scale.

(2) check whether the transmission box in the connection parts are loose, if there is loose, should be tightened one by one.

4. Causes of not discharging or insufficient discharging and elimination methods

(1) broken material or insufficient feed, the equipment in the process of operation must ensure that the feed is sufficient.

(2) The viscosity of the material is too large, if this happens, it is necessary to use pressure feeding or dilute the material.

(3) the main motor drive belt slippage; need to adjust the belt tightening.

(4) plunger seal leakage, need to replace the seal, such as the plunger wear need to replace the plunger.

(5) liquid flow end of the spool valve seat wear, then need to change the spool valve seat.

(6) the liquid flow end of the six groups of spool valve seat damage or impurities stuck in the seal line, should check the damage and timely replacement, the replacement parts can be ground according to the damage back to use or scrap.

5. Pressure gauge pointer swing greater than plus or minus two frames

(1) All the reasons in 5-4 will cause the pressure gauge pointer swing increase.

(2) Plunger and connecting rod coupling is loose, need to re-fix the coupling.

(3) Loose coupling between connecting rod and crosshead, need to retighten set screw.

(4) The amount of oil in the pressure gauge seat is not enough, press the plunger in the seat to the bottom, and then fill up the oil.

(5) Homogeneous valve is seriously damaged, need to replace the homogeneous valve.

(6) The coupling of connecting rod or plunger is loose.

(7) The hydraulic end (pump body) in six groups of spool, valve seat has one or more groups of stuck.

(8) For the valve seat with seal, the seal may be damaged.


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6. No pressure in the pump or the pressure does not go on the causes and troubleshooting methods


(1) No feed.

(2) the main motor drive belt is loose, need to tighten the belt.

(3) If the process of regulating pressure, the main motor current value of the response is normal, then it may be: the pressure gauge seat oil leakage in the end, should be filled with oil or pressure gauge is damaged, need to replace the pressure gauge.

(4) homogeneous valve seal impurities or homogeneous valve damage; then need to check and clean the homogeneous valve, if damaged need to be replaced.

(5) hydraulic end of the spool seat damage or seat seal failure; need to replace the spool valve seat or replace the seal ring.

7. Pressure gauge pointer does not return to zero after pressure relief

(1) If the pressure gauge pointer does not return to zero after shutdown, the pressure gauge is damaged, need to replace the pressure gauge.

(2) regulating the top rod seal ring damage or too tight, need to replace the seal ring or adjust the clearance.





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